In-vitro to in-vivo extrapolation to predict fish growth
2015
Eawag, Swiss Federal Institute of Aquatic Science and Technology, Dübendorf, Switzerland
This study uses a fish cell line combined with mechanism-based computational models, to quantitatively predict chemical impact on fish growth, which is a common endpoint for chronic toxicity testing.
The rainbow trout gill cell line RTgill-W1 and the two fungicides cyproconazole and propiconazole were used for in vitro tests. A physiologically based toxicokinetic (PBTK) model, which simulates a chemical’s distribution into various fish tissues and organs, was used to calculate the chemicals' concentration in the exposure medium. Cell survival and proliferation were measured for up to 120 hours. Inhibition of cell population growth was compared with the inhibition of fish growth, for which data was derived from literature.
In summary, the model, which predicts reduced fish growth based on inhibition of fish cell growth, shows good agreement with literature-derived in vivo data. The method is simple, inexpensive, and rapid, requiring only in-vitro data to calibrate the model.
Toxicology across scales: Cell population growth in vitro predicts reduced fish growth
Julita Stadnicka-Michalak
Added on: 04-15-2024
[1] https://www.science.org/doi/10.1126/sciadv.1500302
