Improved resolution of cryo-electron microscopy
2020
Los Alamos National Laboratory, Los Alamos, USA
The authors improved the quality of the 3D molecular structure maps generated with cryo-electron microscopy by using a density modification method. The theoretical basis of the method is the same as one that has been used in the past to improve structural images from X-ray crystallography. The density-modification procedure was applied to 104 datasets and improved the correlation between the experimental maps and the known atomic structure, increasing the visibility of details in many of the maps.
Improvement of cryo-EM maps by density modification
Thomas C. Terwilliger
Added on: 10-15-2020
[1] https://www.nature.com/articles/s41592-020-0914-9[2] https://www.bionity.com/en/news/1168208/new-algorithm-sharpens-focus-of-world-s-most-powerful-microscopes.html